First dendro-archaeological evidence of a completely excavated medieval settlement in the extreme north of Western Siberia

The Buchta Nakhodka settlement is the only archaeological site in the northern part of Western Siberia that has been fully excavated. This well-preserved settlement on Russia’s Yamal Peninsula around 67° N and 72° E, however, has not yet been calendar dated, and the cultural identity of its inhabitants still remains unknown. Here we apply, for the first time, dendrochronological techniques to absolutely date 13 of the most important archaeological timbers from the Buchta Nakhodka settlement. Statistical robust cross-dating results place the construction into the second quarter of the 13th century. The Buchta Nakhodka settlement reveals no obvious connection with the modern, nomadic population of Yamal, the Nenets. Together with a careful literature review, this study indicates that the closest construction techniques and subsequent settlement forms can be found on Iceland and across northern Fennoscandia, where Sami people are building their houses in a similar way. Our combined archaeological, dendrochronological, ethnographical and (archaeo)zoological evidence suggests that the ancient inhabitants of Buchta Nakhodka were closely related to the Sihirtya people that were often mentioned in ancient Nenets folklore as legendary people who inhabited the Yamal Peninsula previously. In providing unique insights into the medieval settlement history of the northern part of Western Siberia, we hope to encourage further interdisciplinary research projects to be launched at Eurasia’s high-northern latitudes.     

Climate increases regional tree-growth variability in Iberian pine forests

Tree populations located at the geographical distribution limit of the species may provide valuable information about tree‐growth response to changes on climatic conditions. We established nine Pinus nigra, 12 P. sylvestris and 17 P. uncinata tree‐ring width chronologies along the eastern and northern Iberian Peninsula, where these species are found at the edge of their natural range. Tree‐growth variability was analyzed using principal component analysis (PCA) for the period 1885–1992. Despite the diversity of species, habitats and climatic regimes, a common macroclimatic signal expressed by the first principal component (PC1) was found. Moreover, considering the PC1 scores as a regional chronology, significant relations were established with Spanish meteorological data. The shared variance held by the tree chronologies, the frequency of narrow rings and the interannual growth variability (sensitivity) increased markedly during the studied period. This shows an enhancement of growth synchrony among forests indicating that climate might have become more limiting to growth. Noticeably, an upward abrupt shift in common variability at the end of the first half of the 20th century was detected. On the other hand, moving‐interval response functions showed a change in the growth–climate relationships during the same period. The relationship between growth and late summer/autumn temperatures of the year before growth (August–September, negative correlation, and November, positive correlation) became stronger. Hence, water stress increase during late summer previous to tree growth could be linked to the larger growth synchrony among sites, suggesting that climate was driving the growth pattern changes. This agrees with the upward trend in temperature observed in these months. Moreover, the higher occurrence of extreme years and the sensitivity increase in the second half of the 20th century were in agreement with an increment in precipitation variability during the growing period. Precipitation variability was positively related to tree‐growth variability, but negatively to radial growth. In conclusion, a change in tree‐growth pattern and in the climatic response of the studied forests was detected since the mid‐20th century and linked to an increase in water stress. These temporal trends were in agreement with the observed increase in warmer conditions and in precipitation variability.     

雷州半岛红树林湿地鸟类多样性

从2000年5月-2003年11月,调查了雷州半岛红树林湿地鸟类.共记录鸟类133种,水鸟和迁徙鸟分别占总记录鸟类数量的81.7%和70.4%.分别有9种、2种、16种和12种鸟被列为中国红皮书保护种、IUCN保护种、CITES保护种和中国国家Ⅱ级保护种;还有63种被列为中日候鸟协定保护种、32种被列为中澳候鸟协定保护种.不同调查地点,鸟类的种类和数量相差较大,高桥鸟类最丰富,记录到83种鸟;五里鸟类种类最少,仅记录到40种鸟,各地鸟类种的相似性很低.环颈鸻(Charadrius alexandrinus)、白腰杓鹬(Numenius arquata)和红嘴鸥(Larus ridibundus)在雷州半岛的种群数量达到国际重要湿地的标准.雷州半岛鸟类种的丰度和多度季节变化明显,冬季种类多、数量丰富,夏季种类少、数量贫乏.冬季和夏季总的鸟类数量(P=0.043)和水鸟数量(P=0.001)差异明显.保护整个沿海红树林湿地是保护雷州半岛红树林湿地鸟类多样性的关键.     

Genotyping by sequencing reveals contrasting patterns of population structure,ecologically mediated divergence,and long‐distance dispersal in North American palms

Comparative studies can provide powerful insights into processes that affect population divergence and thereby help to elucidate the mechanisms by which contemporary populations may respond to environmental change. Furthermore, approaches such as genotyping by sequencing (GBS) provide unprecedented power for resolving genetic differences among species and populations. We therefore used GBS to provide a genomewide perspective on the comparative population structure of two palm genera, Washingtonia and Brahea, on the Baja California peninsula, a region of high landscape and ecological complexity. First, we used phylogenetic analysis to address taxonomic uncertainties among five currently recognized species. We resolved three main clades, the first corresponding to W. robusta and W. filifera, the second to B. brandegeei and B. armata, and the third to B. edulis from Guadalupe Island. Focusing on the first two clades, we then delved deeper by investigating the underlying population structure. Striking differences were found, with GBS uncovering four distinct Washingtonia populations and identifying a suite of loci associated with temperature, consistent with ecologically mediated divergence. By contrast, individual mountain ranges could be resolved in Brahea and few loci were associated with environmental variables, implying a more prominent role of neutral divergence. Finally, evidence was found for long‐distance dispersal events in Washingtonia but not Brahea, in line with knowledge of the dispersal mechanisms of these palms including the possibility of human‐mediated dispersal. Overall, our study demonstrates the power of GBS together with a comparative approach to elucidate markedly different patterns of genomewide divergence mediated by multiple effectors.     

Genetic structure analysis of natural <Emphasis Type="Italic">Sargassum muticum</Emphasis> (Fucales,Phaeophyta) populations using RAPD and ISSR markers

Sargassum muticum is important in maintaining the structure and function of littoral ecosystems, and is used in aquaculture and alginate production, however, little is known about its population genetic attributes. In this study, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to investigate the genetic structure of four populations of S. muticum and one outgroup of S. fusiforme (Harv.) Setchell from Shandong peninsula of China. The selected 24 RAPD primers and 19 ISSR primers amplified 164 loci and 122 loci, respectively. Estimates of genetic diversity with different indicators (P%, percentage of polymorphic loci; H, the expected heterozygosity; I, Shannon’s information index) revealed low or moderate level of genetic variations within each S. muticum population, and a high level of genetic differentiations were determined with pairwise unbiased genetic distance (D) and fixation index (F _ST ) among the populations. The Mantel test showed that two types of matrices of D and F _ST were highly correlated whether from RAPD (r = 0.9706, P = 0.009) or ISSR data (r = 0.9161, P = 0.009). Analysis of molecular variance (AMOVA) was conducted to apportion the variations among and within the S. muticum populations. It indicated that variations among populations were higher than those within populations, being 55.82% verse 44.18% by RAPD and 55.21% verse 44.79% by ISSR, respectively. Furthermore, the Mantel test suggested that genetic differentiations among populations were related to the geographical distances (r > 0.6), namely, conformed to the IBD (isolation by distance) model, as expected from UPGMA (unweighted pair group method with arithmetic averages) cluster analysis. On the whole, the high genetic structuring among the four S. muticum populations along the distant locations was clearly indicated in RAPD and ISSR analyses (r > 0.9, P < 0.05) in our study.     

Population genetic structure and diversity of the Apennine endemic stream frog, Rana italica--insights on the Pleistocene evolutionary history of the Italian peninsular biota

For most species in the Western Palaearctic region, southern Mediterranean peninsulas have been identified as major Quaternary refugia and hotspots of intraspecific diversity, and thus, as areas of particular relevance for the conservation of the evolutionary potential. We analysed the patterns of geographical variation among 26 populations of the Italian stream frog, using both nuclear (allozymes) and mitochondrial (partial cytochrome b sequences) markers. Phylogenetic, phylogeographical and population genetic analyses suggested that the species survived the last glacial–interglacial cycles in two distinct refugia, one restricted to the tip of the Calabrian peninsula, at the extreme south of the species' range, the other spanning from central Calabria to central Apennines and showing evidences for further population subdivision therein. Historical demographic tests suggested a significant population expansion from the latter, which most likely began around the last pleniglacial. This expansion would have led to the rapid colonization of the northern Apennines to the north, and to a secondary contact and population admixture with the population from the southern refugium in southern central Calabria. A comparison of the evolutionary history inferred for the Italian stream frog with the data emerging for other codistributed species suggests: (i) the generality of a multiple-refugia scenario for the Italian peninsula, (ii) the possible occurrence of at least one suture zone in southern Italy, and (iii) that for most species, this Pleistocene refugium is not only a hotspot, but also a melting pot of intraspecific genetic diversity. Finally, the conservation implications of these results are also briefly highlighted.     

Molecular phylogeography of the nose-horned viper (Vipera ammodytes, Linnaeus (1758)): evidence for high genetic diversity and multiple refugia in the Balkan peninsula

The nose-horned viper (Vipera ammodytes) occurs in a large part of the south-eastern Europe and Asia Minor. Phylogenetic relationships were reconstructed for a total of 59 specimens using sequences from three mitochondrial regions (16S and cytochrome b genes, and control region, totalling 2308 bp). A considerable number of clades were observed within this species, showing a large genetic diversity within the Balkan peninsula. Splitting of the basal clades was evaluated to about 4 million years ago. Genetic results are in contradiction with presently accepted taxonomy based on morphological characters: V. a. gregorwallneri and V. a. ruffoi do not display any genetic difference compared with the nominotypic subspecies (V. a. ammodytes), involving that these subspecies can be regarded as synonyms. High genetic divergence in the central part of the Balkan peninsula is not concordant with low morphological differentiation. Finally, the extensive genetic diversity within the Balkan peninsula and the colonisation routes are discussed.     

Speciation of Iberian diving beetles in Pleistocene refugia (Coleoptera, Dytiscidae)

The Mediterranean basin is an area of high diversity and endemicity, but the age and origin of its fauna are still largely unknown. Here we use species-level phylogenies based on approximately 1300 base pairs of the genes 16S rRNA and cytochrome oxidase I to establish the relationships of 27 of the 34 endemic Iberian species of diving beetles in the family Dytiscidae, and to investigate their level of divergence. Using a molecular clock approach, 18-19 of these species were estimated to be of Pleistocene origin, with four to six of them from the Late Pleistocene ( approximately 100 000 years). A second, lower speciation frequency peak was assigned to Late Miocene or Early Pliocene. Analysis of the distributional ranges showed that endemic species placed in the tip nodes of the trees are significantly more likely to be allopatric with their sisters than endemic species at lower node levels. Allopatric sister species are also significantly younger than sympatric clades, in agreement with an allopatric mode of speciation and limited subsequent range movement. These results strongly suggest that for some taxa Iberian populations were isolated during the Pleistocene long enough to speciate, and apparently did not expand their ranges to recolonize areas north of the Pyrenees. This is in contradiction to observations from fossil beetles in areas further north, which document large range movements associated with the Pleistocene glacial cycles hypothesized to suppress population isolation and allopatric speciation.     

Freshwater molluscs of Yemen Arab Republic

A list is provided of the extant freshwater molluscan fauna of Yemen Arab Republic, based on collections made in wadis, brooks, and wells. A total of 12 freshwater snail species is recorded, some of which serve as intermediate hosts for Trematodes of the genera Schistosoma and Fasciola.